@article{akalestou_intravital_2021, title = {Intravital imaging of islet Ca2+ dynamics reveals enhanced β cell connectivity after bariatric surgery in mice}, volume = {12}, issn = {2041-1723}, url = {https://www.nature.com/articles/s41467-021-25423-8}, doi = {10.1038/s41467-021-25423-8}, abstract = {Abstract Bariatric surgery improves both insulin sensitivity and secretion and can induce diabetes remission. However, the mechanisms and time courses of these changes, particularly the impact on β cell function, are difficult to monitor directly. In this study, we investigated the effect of Vertical Sleeve Gastrectomy ({VSG}) on β cell function in vivo by imaging Ca 2+ dynamics in islets engrafted into the anterior eye chamber. Mirroring its clinical utility, {VSG} in mice results in significantly improved glucose tolerance, and enhanced insulin secretion. We reveal that these benefits are underpinned by augmented β cell function and coordinated activity across the islet. These effects involve changes in circulating {GLP}-1 levels which may act both directly and indirectly on the β cell, in the latter case through changes in body weight. Thus, bariatric surgery leads to time-dependent increases in β cell function and intra-islet connectivity which are likely to contribute to diabetes remission.}, pages = {5165}, number = {1}, journaltitle = {Nature Communications}, shortjournal = {Nat Commun}, author = {Akalestou, Elina and Suba, Kinga and Lopez-Noriega, Livia and Georgiadou, Eleni and Chabosseau, Pauline and Gallie, Alasdair and Wretlind, Asger and Legido-Quigley, Cristina and Leclerc, Isabelle and Salem, Victoria and Rutter, Guy A.}, urldate = {2021-11-01}, date = {2021-12}, langid = {english}, keywords = {{WP}4}, }