TY - JOUR TI - Regulated expression and function of the GABAB receptor in human pancreatic beta cell line and islets AU - Rachdi, Latif AU - Maugein, Alicia AU - Pechberty, Severine AU - Armanet, Mathieu AU - Hamroune, Juliette AU - Ravassard, Philippe AU - Marullo, Stefano AU - Albagli, Olivier AU - Scharfmann, Raphael T2 - Scientific Reports AB - Abstract G protein-coupled receptors are seven transmembrane signaling molecules that are involved in a wide variety of physiological processes. They constitute a large protein family of receptors with almost 300 members detected in human pancreatic islet preparations. However, the functional role of these receptors in pancreatic islets is unknown in most cases. We generated a new stable human beta cell line from neonatal pancreas. This cell line, named ECN90 expresses both subunits ( GABBR1 and GABBR2 ) of the metabotropic GABA B receptor compared to human islet. In ECN90 cells, baclofen, a specific GABA B receptor agonist, inhibits cAMP signaling causing decreased expression of beta cell-specific genes such as MAFA and PCSK1, and reduced insulin secretion. We next demonstrated that in primary human islets, GABBR2 mRNA expression is strongly induced under cAMP signaling, while GABBR1 mRNA is constitutively expressed. We also found that induction and activation of the GABA B receptor in human islets modulates insulin secretion. DA - 2020/12// PY - 2020 DO - 10.1038/s41598-020-69758-6 DP - DOI.org (Crossref) VL - 10 IS - 1 SP - 13469 J2 - Sci Rep LA - en SN - 2045-2322 UR - https://www.nature.com/articles/s41598-020-69758-6 Y2 - 2021/10/26/12:49:29 KW - WP5 ER -